NEW PRODUCTReliance SARS-CoV-2 RT-PCR Assay Kit
Fast, reliable detection of multiple RNA targets from the SARS-CoV-2 virus.

Bio-Rad's high-performance PCR amplification systems feature the latest technological advances, providing greater accuracy and reproducibility in nucleic acid amplification for genomic experiments. Product lines include thermal cyclers, real-time PCR systems, PCR reagents, plastic products, and NGS library prep kits, as well as our ddPCR and ddSEQ Systems.
NEW PRODUCTIntroducing the new CFX Opus Real-Time PCR System
The new CFX Opus System puts you on the road to integrated workflow for the modern lab. CFX Opus System is the next step in connectivity and usability from your trusted qPCR partner.
PCR Systems

Digital PCR
Bio-Rad's unique Droplet Digital PCR technology provides absolute quantification of nucleic acids for a wide range of applications including cancer mutation studies, HIV quantification, and environmental monitoring.
View ddPCR Products | Find Your ddPCR Assay
qPCR Detection Systems
These systems deliver sensitive, reliable detection of both singleplex and multiplex real-time PCR reactions.
Traditional PCR Systems
DNA amplification instruments range from personal thermal cyclers to the flexible 1000-series.

qPCR Analysis Software
CFX Maestro Software is designed to streamline data collection, visualization, and analysis. Precision Melt Analysis software enables high resolution melt for SNP Genotyping and detection of small indels.
Firmware & Software Updates
Software and firmware for Bio-Rad thermal cyclers and real-time PCR systems are periodically updated. Download updates and instructions here.

CFX Automation System II
The CFX Automation System II works with all CFX Real-Time PCR Systems to facilitate automation of workflows, generation of large volumes of data, and rapid data analysis.

PCR Reagents, Consumables & Accessories
PCR & qPCR Reagents
A wide range of reagents for reverse transcription, PCR, and real-time PCR, optimized to generate accurate and reproducible data.
PrimePCR PCR Primers, Assays & Arrays
Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection analysis for real-time PCR and Droplet Digital PCR.
PCR Plastic Consumables
A large selection of thin-wall PCR tubes, plates, seals, and accessories precisely manufactured for optimal fit and cycling performance in Bio-Rad thermal cyclers, real-time PCR systems, ddPCR systems, and all major competitor systems.
Selector Tools
Use our selectors to find the best reagents, plates, tubes, seals, and accessories for your PCR and qPCR experiments.


PX1 PCR Plate Sealer
Efficiently seals microplates for PCR amplification to deliver reliable data by minimizing sample evaporation during thermal cycling.
In Vitro Diagnostics (IVD)

Reliance SARS-CoV-2 RT-PCR Assay Kit
The Bio-Rad Reliance SARS-CoV-2 RT-PCR Assay Kit is a molecular in vitro diagnostic test containing the reagents required to perform a RT-PCR test. The primer and probe sets are designed to detect RNA from the SARS-CoV-2 virus in upper respiratory tract specimens.
- Highly sensitive one-step RT-PCR workflow
- Specific panel designed with three sets of primers/probes in a single optimized assay multiplex
- Convenient kit package with all the reagent components including positive and negative controls, at a single storage temperature of –20ºC
- Compatible on the most popular PCR instrument platforms (CFX and AB7500 Systems)
CFX96 In Vitro Diagnostics (IVD) Real-Time PCR Systems
CFX96 In Vitro Diagnostics (IVD) Real-Time PCR Systems combine the flexibility you need in your workflow and outstanding performance to deliver reliable results that your clinical laboratory demands.
Zika, Dengue, and Chikungunya (ZDC) Real-Time PCR Assays
Bio-Rad Laboratories developed the ZDC Multiplex RT-PCR Assay (Research Use Only) for the simultaneous detection of Zika, dengue, and chikungunya viral RNA.

SEQuoia Complete Stranded RNA Library Prep Kit
High-performance RNA-Seq kit that captures long and short RNAs in a single library.
Next-Generation Sequencing
To assist researchers in answering the most complex biological questions, Bio-Rad offers a number of novel products for NGS library preparation using cutting-edge technologies, such as whole transcriptome and single-cell sequencing solutions.
lncRNA RT-qPCR Workflow

-
1 RT and Preamplification
-
2 qPCR
-
3 Data Analysis
Bio-Rad's innovative workflow enables highly sensitive detection of long noncoding RNA (lncRNA) and helps to overcome challenges associated with IncRNA expression analysis.
Learn More
Nucleic Acid Electrophoresis & Blotting
Bio-Rad's selection of electrophoresis and blotting instruments for nucleic acids includes submerged horizontal and vertical cells in various sizes, PFGE systems, and solutions for sequencing and mutation detection.
Nucleic Acid Sample Preparation
RNA Isolation
A wide range of kits and reagents for the isolation of highly pure RNA from different starting materials.
DNA Isolation
Various kits for the extraction of plasmid and PCR-ready genomic DNA from a variety of starting materials.
DNA Cleanup
A variety of solutions for purifying DNA from various reaction mixtures or extracting DNA fragments from agarose gels.

Kit | DNA Yield |
Preparation Time |
Bacterial Culture Density or Volume |
Purification Format |
Binding Matrix |
Growth Media |
|
---|---|---|---|---|---|---|---|
Aurum plasmid mini | ![]() |
>20µg | 10 min | Up to 12 OD • ml* | Vacuum** and spin |
Silica membrane |
Standard |
Quantum Prep miniprep | ![]() |
>20µg | 15 min | 1–10 ml | Spin | Diatomaceous earth |
Enriched or standard |
* (OD600 of undiluted culture) x (culture volume in ml) = #of OD • ml. 1 OD600 is equivalent to approximately 8 x 108 cells/ml.
** Using the Aurum vacuum manifold.
Aurum Total RNA Kits | PureZOL RNA Isolation Reagent |
|||
---|---|---|---|---|
Mini Kit | Fatty and Fibrous Tissue Kit |
96 Kit | ||
Format | Mini column Filtration (vacuum or spin) | Mini column Filtration (vacuum or spin) | 96-well plate Filtration (vacuum or spin) | — |
Maximum starting amounts | ||||
Cultured cells | 2 x 106 | — | 1 x 106 | 1 x 107 |
Bacterial cells | 2.4 x 109 | — | 8 x 108 | 2.4 x 109 |
Yeast cells | 3 x 107 | — | 2 x 107 | 3 x 107 |
Animal tissue — hard | 20 mg | 100 mg | — | 100 mg |
Animal tissue — soft to moderately hard |
40 mg | 100 mg | — | 100 mg |
Plant tissue | 40 mg | 100 mg | — | 100 mg |
Isolation method | Silica membrane | Lysis with PureZOL Reagent, purification on silica membrane | Silica membrane | Organic extraction |
Number of preps | 50 mini preps | 50 mini preps | 2 x 96-well plate | 50 or 100 preps (1 ml/prep) |
Number of washes | 3 | 3 | 3 | — |
DNase I included* | Yes | Yes | Yes | No |
DNase I digest time | 15 min (animal tissue, 25 min) | 15 min | 10 min | — |
Total preparation time | <50 min (1.5 hr with DNase I) | <50 min (1.5 hr with DNase I) | <60 min | <60 min |
Binding capacity | >100 µg | >100 µg | >40 µg | — |
Elution volume | 2 x 40 µl | 2 x 40 µl | 80 µl | 30–100 µl |
Yield** | 28 µg/2 x 106 NIH 3T3 cells | 96 µg/100 mg brain | 10 µg/1 x 106 NIH 3T3 cells | 1.15 µg/mg brain |
38 µg/2 x 106 HeLa cells | 58 µg/100 mg breast | 17 µg/1 x 106 HeLa cells | 1.37 µg/mg fat | |
20 µg/40 mg brain | 85 µg/100 mg heart | 11 µg/2 x 107 S. cerevisiae cells | 1.2 µg/mg heart | |
52 µg/20 mg thymus | 63 µg/100 mg skin | 5 µg/8 x 108 E. coli cells | 0.66 µg/mg cartilage | |
132 µg/40 mg spleen | 54 µg/100 mg cartilage | 5 µg/8 x 108 B. cereus cells | 1.2 µg/mg skin | |
36 µg/40 mg lung | 1.0 µg/mg potato | |||
32 µg/40 mg potato | 1.37 µg/1 x 105 293H cells | |||
28 µg/3 x 107 S. cerevisiae cells | 0.1 µg/mg Arabidopsis | |||
54 µg/2.4 x 109 E. coli cells | 0.38 µg/mg A. niger | |||
0.63 µg/2.4 x 107 E. coli cells | ||||
0.31 µg/3 x 105 S. cerevisiae cells | ||||
Purity | A260/A280 of 1.9–2.1 | A260/A280 of 1.9–2.1 | A260/A280 of 1.9–2.1 | A260/A280 of 1.9–2.1 |
* Removal not required.
** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.
Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.
Freeze 'N Squeeze DNA Gel Extraction Spin Columns |
Micro Bio-Spin and Bio-Spin Columns | PCR Kleen Spin Columns | |
---|---|---|---|
Application | Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels | Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting | Removal of PCR products >200 bp from other reaction mixture components |
Starting Material | ≤700 mg gel slice | 10–100 µl of sequencing reaction mixture | 25–100 µl of PCR or other enzyme reaction |
Format | Spin-column extraction of frozen-thawed gel slices | Prepacked size exclusion spin column | Prepacked size exclusion spin column |
Recovery | 4–23 kb: >80% recovery; 0.5–4 kb: >70% recovery; 50–500 bp: >50% recovery |
>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators | 50–80% |
Time Required | <10 min | <10 min | <4 min |
Resources
Applications & Technologies
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
A snapshot of the critical steps needed to achieve excellent qPCR results.
Tips, Tricks & Best Practices for qPCR
The Ultimate qPCR Guide.
What is Real-Time PCR (qPCR)?
Learn about the proper design, optimization, analysis, and troubleshooting of real-time polymerase chain reactions.
Digital PCR and Real-Time PCR (qPCR) Choices for Different Applications
Compare the capabilities, advantages, and main applications of digital PCR and qPCR technologies.
Real-Time PCR Troubleshooting
Find possible causes and treatments for common qPCR problems.
Introduction to qPCR Instrumentation
Learn about the different Bio-Rad real-time PCR instruments and software available for data analysis.
MIQE and RDML Guidelines
Become familiar with the minimum information guidelines required to publish qPCR experiments and with RDML.

Service & Support
Thermal Validation Services
Bio-Rad's Thermal Validation Services ensure that your thermal cyclers and real-time PCR instruments are performing optimally.
IQ/OQ Services
Installation Qualification (IQ) and Operational Qualification (OQ) Services for thermal cyclers and real-time PCR systems ensure that your systems function properly.
CFX Qualification Plate
Use the predispensed CFX Qualification Plate to quickly and easily verify the performance of your CFX Real-Time PCR Detection System.
Contact an Expert
Consult with a specialist about how Bio-Rad can help you with your genomics research.
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