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|PCR Systems||PCR Reagents, Consumables & Accessories||In Vitro Diagnostics (IVD)||Next-Generation Sequencing||lncRNA RT-qPCR Workflow||Nucleic Acid Electrophoresis & Blotting||Nucleic Acid Sample Preparation||Resources|
Bio-Rad's high-performance PCR amplification systems feature the latest technological advances, providing greater accuracy and reproducibility in nucleic acid amplification for genomic experiments. Product lines include thermal cyclers, real-time PCR systems, PCR reagents, plastic products, and NGS library prep kits, as well as our ddPCR and ddSEQ Systems.
Bio-Rad's unique Droplet Digital PCR technology provides absolute quantification of nucleic acids for a wide range of applications including cancer mutation studies, HIV quantification, and environmental monitoring.
CFX Maestro Software is designed to streamline data collection, visualization, and analysis. Precision Melt Analysis software enables high resolution melt for SNP Genotyping and detection of small indels.
Software and firmware for Bio-Rad thermal cyclers and real-time PCR systems are periodically updated. Download updates and instructions here.
The CFX Automation System II works with all CFX Real-Time PCR Systems to facilitate automation of workflows, generation of large volumes of data, and rapid data analysis.
PCR Reagents, Consumables & Accessories
A wide range of reagents for reverse transcription, PCR, and real-time PCR, optimized to generate accurate and reproducible data.
Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection analysis for real-time PCR and Droplet Digital PCR.
A large selection of thin-wall PCR tubes, plates, seals, and accessories precisely manufactured for optimal fit and cycling performance in Bio-Rad thermal cyclers, real-time PCR systems, ddPCR systems, and all major competitor systems.
Use our selectors to find the best reagents, plates, tubes, seals, and accessories for your PCR and qPCR experiments.
Efficiently seals microplates for PCR amplification to deliver reliable data by minimizing sample evaporation during thermal cycling.
In Vitro Diagnostics (IVD)
The Bio-Rad Reliance SARS-CoV-2 RT-PCR Assay Kit is a molecular in vitro diagnostic test containing the reagents required to perform a RT-PCR test. The primer and probe sets are designed to detect RNA from the SARS-CoV-2 virus in upper respiratory tract specimens.
- Highly sensitive one-step RT-PCR workflow
- Specific panel designed with three sets of primers/probes in a single optimized assay multiplex
- Convenient kit package with all the reagent components including positive and negative controls, at a single storage temperature of –20ºC
- Compatible on the most popular PCR instrument platforms (CFX and AB7500 Systems)
CFX96 In Vitro Diagnostics (IVD) Real-Time PCR Systems combine the flexibility you need in your workflow and outstanding performance to deliver reliable results that your clinical laboratory demands.
Bio-Rad Laboratories developed the ZDC Multiplex RT-PCR Assay (Research Use Only) for the simultaneous detection of Zika, dengue, and chikungunya viral RNA.
To assist researchers in answering the most complex biological questions, Bio-Rad offers a number of novel products for NGS library preparation using cutting-edge technologies, such as whole transcriptome and single-cell sequencing solutions.
lncRNA RT-qPCR Workflow
1 RT and Preamplification
3 Data Analysis
Bio-Rad's innovative workflow enables highly sensitive detection of long noncoding RNA (lncRNA) and helps to overcome challenges associated with IncRNA expression analysis.Learn More
Nucleic Acid Electrophoresis & Blotting
Bio-Rad's selection of electrophoresis and blotting instruments for nucleic acids includes submerged horizontal and vertical cells in various sizes, PFGE systems, and solutions for sequencing and mutation detection.
Nucleic Acid Sample Preparation
A wide range of kits and reagents for the isolation of highly pure RNA from different starting materials.
Various kits for the extraction of plasmid and PCR-ready genomic DNA from a variety of starting materials.
A variety of solutions for purifying DNA from various reaction mixtures or extracting DNA fragments from agarose gels.
|Aurum Total RNA Kits||PureZOL RNA
|Mini Kit||Fatty and Fibrous
|Format||Mini column Filtration (vacuum or spin)||Mini column Filtration (vacuum or spin)||96-well plate Filtration (vacuum or spin)||—|
|Maximum starting amounts|
|Cultured cells||2 x 106||—||1 x 106||1 x 107|
|Bacterial cells||2.4 x 109||—||8 x 108||2.4 x 109|
|Yeast cells||3 x 107||—||2 x 107||3 x 107|
|Animal tissue — hard||20 mg||100 mg||—||100 mg|
| Animal tissue — soft
to moderately hard
|40 mg||100 mg||—||100 mg|
|Plant tissue||40 mg||100 mg||—||100 mg|
|Isolation method||Silica membrane||Lysis with PureZOL Reagent, purification on silica membrane||Silica membrane||Organic extraction|
|Number of preps||50 mini preps||50 mini preps||2 x 96-well plate||50 or 100 preps (1 ml/prep)|
|Number of washes||3||3||3||—|
|DNase I included*||Yes||Yes||Yes||No|
|DNase I digest time||15 min (animal tissue, 25 min)||15 min||10 min||—|
|Total preparation time||<50 min (1.5 hr with DNase I)||<50 min (1.5 hr with DNase I)||<60 min||<60 min|
|Binding capacity||>100 µg||>100 µg||>40 µg||—|
|Elution volume||2 x 40 µl||2 x 40 µl||80 µl||30–100 µl|
|Yield**||28 µg/2 x 106 NIH 3T3 cells||96 µg/100 mg brain||10 µg/1 x 106 NIH 3T3 cells||1.15 µg/mg brain|
|38 µg/2 x 106 HeLa cells||58 µg/100 mg breast||17 µg/1 x 106 HeLa cells||1.37 µg/mg fat|
|20 µg/40 mg brain||85 µg/100 mg heart||11 µg/2 x 107 S. cerevisiae cells||1.2 µg/mg heart|
|52 µg/20 mg thymus||63 µg/100 mg skin||5 µg/8 x 108 E. coli cells||0.66 µg/mg cartilage|
|132 µg/40 mg spleen||54 µg/100 mg cartilage||5 µg/8 x 108 B. cereus cells||1.2 µg/mg skin|
|36 µg/40 mg lung||1.0 µg/mg potato|
|32 µg/40 mg potato||1.37 µg/1 x 105 293H cells|
|28 µg/3 x 107 S. cerevisiae cells||0.1 µg/mg Arabidopsis|
|54 µg/2.4 x 109 E. coli cells||0.38 µg/mg A. niger|
|0.63 µg/2.4 x 107 E. coli cells|
|0.31 µg/3 x 105 S. cerevisiae cells|
|Purity||A260/A280 of 1.9–2.1||A260/A280 of 1.9–2.1||A260/A280 of 1.9–2.1||A260/A280 of 1.9–2.1|
Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.
|Freeze 'N Squeeze DNA
Gel Extraction Spin Columns
|Micro Bio-Spin and Bio-Spin Columns||PCR Kleen Spin Columns|
|Application||Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels||Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting||Removal of PCR products >200 bp from other reaction mixture components|
|Starting Material||≤700 mg gel slice||10–100 µl of sequencing reaction mixture||25–100 µl of PCR or other enzyme reaction|
|Format||Spin-column extraction of frozen-thawed gel slices||Prepacked size exclusion spin column||Prepacked size exclusion spin column|
|Recovery||4–23 kb: >80% recovery;
0.5–4 kb: >70% recovery;
50–500 bp: >50% recovery
|>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators||50–80%|
|Time Required||<10 min||<10 min||<4 min|
Applications & Technologies
A snapshot of the critical steps needed to achieve excellent qPCR results.
The Ultimate qPCR Guide.
Learn about the proper design, optimization, analysis, and troubleshooting of real-time polymerase chain reactions.
Compare the capabilities, advantages, and main applications of digital PCR and qPCR technologies.
Find possible causes and treatments for common qPCR problems.
Learn about the different Bio-Rad real-time PCR instruments and software available for data analysis.
Become familiar with the minimum information guidelines required to publish qPCR experiments and with RDML.
Service & Support
Bio-Rad's Thermal Validation Services ensure that your thermal cyclers and real-time PCR instruments are performing optimally.
Installation Qualification (IQ) and Operational Qualification (OQ) Services for thermal cyclers and real-time PCR systems ensure that your systems function properly.
Use the predispensed CFX Qualification Plate to quickly and easily verify the performance of your CFX Real-Time PCR Detection System.
Consult with a specialist about how Bio-Rad can help you with your genomics research.